Neural cell adhesion molecule ncam, also called cd56, is a homophilic binding glycoprotein expressed on the surface of neurons, glia and skeletal muscle. Cd56 expression predicts occurrence of cns disease in. Jul 01, 2003 expression of cd56 on maturing myeloid cells or monocytes. The aim of the study is to determine the prognostic relevance of cd200 cd56 expression in adult acute lymphoblastic leukemia patients. A novel myeloidlike nk cell progenitor in human umbilical cord blood. Jul 26, 2001 cd56 antigen, a 200220 kda cell surface glycoprotein, identified as an isoform of the neural adhesion molecules ncam, has been found frequently expressed in several lymphohematopoietic neoplasms including acute myeloid leukemias aml. Cd56 is a marker for natural killer nk cells, but is also expressed in neoplastic myeloid, lymphoid, plasmacytoid dendritic and myeloma cells, as well as in a minority of t cells. Aberrant cd56 expression is identified in most patients with myeloma. Tcell acute lymphoblastic leukemia all is an aggressive hematological malignancy, accounting for 25% of all adult cases of all. Tcell acute lymphoblastic leukemia with coexpression of. Compared with amlnos, granulocytic cells in amlmrc had higher cd33 expression but lower cd45, cd11b and cd15.
However, expression of cd7, cd33, cd34, cd56, neic bone marrow transplantation, most persued fatal and frequently hladr, but not other nk, t cell, and b cell courses within 41 months. These immature cells have the capacity to suppress immune responses mediated by natural. The expression of cd56 is considered a bad prognostic factor for overall survival, lower rates or short complete remission and extramedullary invasion but the results are controversial. Cd56 expression in myeloperoxidasenegative fab m5 acute myeloid leukemia. These data suggested that the markers was observed. Coexpression of myeloid markers together with cd34 and cd56 in tcell all is seen more frequently 6, 7. Pdf cd56 and cd19 antigens expression in acute myeloid. Monoclonality was ascertained on tcr gene rearrangements by pcr. Contributes to cellcell or cellmatrix adhesion during development. Cd56 expression is associated with extramedullary leukemic infiltration. The death rate during induction was not significantly different despite an apparent difference in proportions between groups. Cd56 antigen, a 200220 kda cell surface glycoprotein, identified as an isoform of the neural adhesion molecules ncam, has been found. Jan 01, 2011 the diagnostic criteria should include the following. Ocular manifestation in myeloidnk cell precursor acute leuk.
Cd56 expression on human immune cells in health and disease. Kazuo muroi, shinichiro fujiwara, raine tatara, miyuki sugimoto, chihiro yamamoto, eisuke uehara, akiko meguro, kaoru hatano, kiyoshi okazuka, iekuni oh, ken ohmine, takahiro suzuki, masaki mori, tadashi nagai, keiya ozawa pmid 24369228. Cd56 marks human dendritic cell subsets with cytotoxic. Nov 16, 2010 the data suggest that expression of cd56 in acute myeloid leukemia may be indicative of poor prognosis because it is associated with a shorter overall survival. Diagnostic confusion resulting from cd56 expression by. Cd56 antigenic expression in acute myeloid leukemia identifies. Cd56 antigenic expression in acute myeloid leukaemia identifies patients. The prognostic implications of cd56 expression in aml are not clear. Feb 01, 20 despite its stringent association with nk cells, the expression of cd56 has also been detected on other human lymphoid cells including nkt cells and activated t cells. Hematological malignancies with cd56 expression mainly arise from nk or nklike cells. Cd56 can also be expressed in certain neoplastic cells such as aml cells, acute myeloidnk cell leukemia myeloidnk al, all and lymphoma. The coexpression of the myeloid dc lineage markers bdca1 and cd11c along with the absence of cd7 expression, which allows their accurate discrimination from nk cells 26,35, lends further support to the notion that il15 dcs are unrelated to nk cells in spite of their partial positivity for cd56. Cd56 expression in acute myeloid leukemia aml has been associated with extramedullary leukemia and multidrug resistance, but its clinical and prognostic significance has not been clearly identified. The diagnosis of myeloid neoplasia by flow cytometry.
Review of the relevance of aberrant antigen expression by. Neural cell adhesion molecule cd56positive b cell lymphoma. Cd56 is a neural cell adhesion molecule isoform that plays an important role in migration through cell to cell adhesion via homophilic adhesion. The main goal of the present study is to investigate cd200 and cd56 aberrant expression in cd34positive blasts, in newly diagnosed adult aml patients and. We herein report a case of t cell all exhibiting aberrant cd34, cd56, cd33 and cd117 expression in addition to t. In bone marrow, there are two subsets of plasma cells. Overexpression of pglycoprotein pgp, a multidrugrelated mdr protein, is one of the most important factors responsible for reduced drug sensitivity in acute myeloid leukemia aml. Cd56 marks human dendritic cell subsets with cytotoxic potential. Delineation of natural killer cell differentiation from myeloid progenitors in human. Cd56 expression on the membrane of monocytes, suggesting distinct. Cornbleet, oral personal communication, february 1996. The expression of cd56 is a rare phenomenon in lymphoid malignancies, mostly confined to those of t nk cell origin, 58 generally characterized by unique extranodal involvement, with lesions in the nasalparanasal region, skin, lung, and stomach, and often display the angiocentric growth pattern. Nk maturation begins with the expression of cd56 followed by simultaneous expression of cd94nkg2a.
Acute myeloid leukemia, cd79a aberrant expression, cd56 and cd7. Prognostic impact of cd200 and cd56 expression in adult. Dendritic cell nk cell stem cell precursor macrophage monocyte granulocyte platelet erythrocyte endothelial cell epithelial cell key markers human cd11c cd123 cd56 cd34 hematopoetic stem cell only cd14 cd33 cd66b cd41 cd61 cd62 cd235a cd146 cd326 key markers mouse b cell cd19 cd20 cd45rb220 cd19 cd22 b cell activation marker t cell cd3. Quantitative multiplex immunohistochemistry reveals myeloid. In this study, we evaluated the correlation among cd56 expression, cytogenetic abnormality, and clinical outcome in aml. Potently cytotoxic natural killer cells initially emerge. Recently, we demonstrated that the presence of cd56 antigen, an. Abnormal expression of cd79a, cd56 and cd7 in acute. Aug 12, 2003 cd56 neural cell adhesive molecule ncam is an often. Cd56 antigen, a 200220 kda cell surface glycoprotein, identified as an isoform of the neural adhesion molecules ncam, has been found frequently expressed in several lymphohematopoietic neoplasms including acute myeloid leukemias aml. Cd56 and cd19 antigens expression in acute myeloid. Abnormal expression of cd79a, cd56 and cd7 in acute myeloid. Moreover, the adverse prognostic implication of cd56 expression on myeloid blast cells was also confirmed by the fact that in univariate statistical analysis the median duration of overall.
The diagnosis of myeloid neoplasia by flow cytometry brent l. Metabolic regulation of myeloidderived suppressor cell. Aberrant expression of cd56 on granulocytes and monocytes. Delineation of natural killer cell differentiation from.
If expressed on myeloblasts it is a predictor of lower complete response rate and overall survival os raspadori et al, 2001, 2002. American journal of respiratory cell and molecular biology. Compared to monocytes, granulocytes had lower frequencies of cd56 expression but higher specificity for abnormal cells. Expression of cd56 was found on a variety of immune cells figure 1. Expression of myeloid antigen in neoplastic plasma cells is. The expression of cd200 and cd56 by blast cells was assessed by flow cytometry before the start of chemotherapy in 70 ball patients. There are a number of reasons that the cd56 expression on nk cells need not impede development and clinical use of a cd56targeted therapy. Aberrant expression of cd56 on granulocytes and monocytes in. Nkp46 and nkg2d receptor expression in nk cells with cd56dim. However, cd56 expression has been reported in acute myeloid or monocytic leukemia, acute promyelocytic leukemia, and myeloma.
Surface cd56 expression on leukemic cells in acute promyelocytic leukemia apml is considered an indicator of poorer outcome even in pati. Acute myeloid leukemia with cd56 expression high ncidence. Neoplastic monoblasts, in particular, can have a similar immunophenotype, including expression of cd123. The expression of cd56 antigen is associated with poor. Cd19 is a blymphocyte marker, whose expression is associated with. Morphologic dysplasia was associated with significantly lower granulocytic forward scatter, side scatter and cd10 but higher cd33 expression. Cd56 and cd57 are known to be markers for natural killer nk cells, with cd56 along with cd16 being a lineage marker for nk cells. The expression of cd56 antigen is associated with poor prognosis. It has been proposed that stage 2 cells, which are precursor nk cells, can traffic to lymph. Evaluation of the expression of cd200 and cd56 in cd34positive adult acute myeloid leukemia and its effect on the response to induction of chemotherapy zainab najah muhsin, subh salem almudallal department of pathology and forensic medicine, college of medicine, alnahrain university, baghdad, iraq. Flow cytometry rapidly identifies all acute promyelocytic. We report a case of myeloid sarcoma in a patient who presented with an ear lobe mass and facial nerve paralysis. When comparing with reactive group, cd56 expression level in the monocytes in all three neoplastic groups was higher, hla. The main goal of the present study is to investigate cd200 and cd56 aberrant expression in cd34positive.
Expression of the adhesion molecule cd56 may occur due to abnormalities in these compartments or because of early or intense granulopoiesis following a marrow insult figure 3ef j. Cd56 expression is an indicator of poor clinical outcome. Cd56 is the archetypal phenotypic marker of natural killer cells but can actually be expressed by many more immune cells, including alpha beta t cells. It should be noted that this immunophenotype, although distinctive, is not completely specific. In this respect, cd56 also known as neural cell adhesion molecule is a very good example.
Cd56 cd34 myeloidlike adherent cell fraction acf from umbilical cord blood. Predictable prognostic factor of cd56 expression in patients with. Cd57 is not usually found in the fetal nk cells, however its expression increases with age 5. Expression of cd56 by the malignant cells led to an initial misdiagnosis as merkel cell tumor. Using a fourcolor flow cytometry panel, harrington et al. In addition to the expression of cd56 on nk cells, cd56 is also expressed on the surface of neurons and glia. First, nk cells turn over approximately every 2 weeks in the circulation. Chronic myeloid leukemia 045708 cml in chronic phase 2. Differential killing of cd56expressing cells by drugconjugated human antibodies. Cd56, the neural cell adhesion molecule ncam, is a member of the immunoglobulin superfamily and is associated with celltocell adhesion and migration. Although cd56 is often considered a marker of neural lineage commitment due to its discovery site, cd56 expression is also found in, among others, the hematopoietic system. Cd56 antigen expression and hemophagocytosis of leukemic. Over the past years, the phenotypic and functional boundaries distinguishing the main cell subsets of the immune system have become increasingly blurred.
Cd56 expression is an independent prognostic factor for. Plasmacytoid dendritic cell an overview sciencedirect topics. Predictable prognostic factor of cd56 expression in. Cytoplasmic cd3 was detected in cd7and cd56 myeloid nk cell precursor acute leukemia. Cd56 expression in normal immature granulocytes after. We investigated the prognostic relevance of cd56 in a series of patients with apl who were treated homogeneously with alltransretinoic acid atra and. Bright cd38 expression with coexpression of cd56 is used to detect abnormal populations of.
Pdf cd56 was firstly described as a marker of natural killer cells and has been found expressed in several neoplasms including acute. Cd56 expression was analyzed in leukemic cells from 94 adults with primary. Cd56 high acute myeloid leukemias amls have a poor prognosis, but it has been unclear how cd56 expression is controlled and how it relates to clinical aggressiveness. The expression status of cd, cd19, cd20, cd33, cd38, cd56, and cd117 was analyzed on myeloma cells from 55 newly diagnosed patients, including 36 men 65%, of median age 61 years range. Monocytes in amlmrc had lower expression of cd14, cd56 and cd45. Immunohistochemical findings in blastic plasmacytoid dendritic cell neoplasm are distinctive. Myeloid and monocytic dyspoiesis as determined by flow.
Here, the latter two populations were further subdivided by examining cells in the r10 gate by their cd3 versus cd56 expression. Using this platform in a head and neck squamous cell carcinoma hnscc cohort, we revealed that differential immune complexity, representing either lymphoid or myeloid in. Human plasmacytoid and myeloid dendritic cells dcs, when appropriately stimulated, can express the archetypal natural killer nkcell surface marker cd56. Cd56, the neural cell adhesion molecule ncam, is a member of the immunoglobulin superfamily and is associated with cell to cell adhesion and migration. Assessment of myeloid and monocytic dysplasia by flow. Both studies used tissue samples of resected tumor or a serous effusion for fc. We herein report a case of tcell all exhibiting aberrant cd34, cd56, cd33 and cd117 expression in addition to t. The authors concluded that fc of cd56 expression by the neoplastic cells identified the cells as neuroectodermal. Akin to the first case, cd7 expression was variable dim to negative, seen in around 30% cells. The expression of cd56 antigen is associated with poor prognosis in patients with acute myeloid leukemia. Myeloidderived suppressor cells myeloidderived suppressor cells mdscs are a heterogeneous population of immature myeloid progenitor cells that fail to differentiate into. Jul 31, 20 in chronic myeloid leukemia without an increase of blasts, aberrant cd56 expression can be assessed using customdesigned antigen panels by paring of cd56 with a stem cell marker. Cd56 is one of the most adverse risk factors in patients with aml 57.
Flow cytometric immunophenotypic study of monocytes in myeloid. Positive refers to any expression of this cd marker either in all cells, a subset, or upon activation. Myeloid sarcomas are tumor masses composed of aggregates of malignant myeloid precursors in extramedullary sites including the skin. Cd7 on the blasts in cml blast crisis as well as cd7 expression on cd34positive cells in chronic phase. We evaluated the association between the expression of myeloid antigens on neoplastic plasma cells and patient prognosis. Novel runx1 isoforms determine the fate of acute myeloid. Preliminary reports suggest that leukemic cell expression of cd56, a neural cell adhesion molecule, is associated with adverse clinical outcome in either acute myeloid leukemia with t8. However, the cells express nkp46, albeit at low levels under certain conditions, and display a variegated expression. Evaluation of the expression of cd200 and cd56 in cd34. Cd56 antigenic expression in acute myeloid leukemia. Myeloid derived suppressor cells mdscs are a heterogeneous population of immature myeloid progenitor cells that fail to differentiate into granulocytes, macrophages, and dendritic cells. Pdf cd56 and pgp expression in acute myeloid leukemia. Cd56 over expression and hladr underexpression are the most common immunophenotypic aberrancies. Prolactin acts on myeloid progenitors to modulate smad7 expression and enhance hematopoietic stem cell differentiation into the nk cell lineage skip to main content thank you for visiting.
Low levels of cd56 expression were detected on the granulocytes in normal bm after chemotherapy and stem cell transplantation, but. Aberrant expression of cd56 with varying frequencies has been recognized in mds, 12 chronic myelomonocytic leukemia, 17 and chronic myelogenous leukemia. Aberrant expression of cd56 on the blasts and myeloid cells. Final nk maturation in the bone marrow correlates with a reduction in cd94nkg2a expression and a cd56low phenotype stage 5b. Over expression of cd22 is common in ball with hyperdiploidy 4. Cd56 antigen expression showed a correlation with the total rate of hemophagocytosis plus vacuolation, with a correlation coefficient of 0. Cd56 expression is an independent prognostic factor for relapse in acute myeloid leukemia with t8. Flowcytometry not only can diagnose acute leukemia from peripheral bloodbone marrow, it can also detect leukemic cells in cerebrospinal fluid to detect central nervous system cns involvement, to start. Prolactin acts on myeloid progenitors to modulate smad7. Potently cytotoxic natural killer cells initially emerge from. Cd56 expression in normal immature granulocytes after allogeneic hematopoietic stem cell transplantation. Nkp46 and nkg2d receptor expression in nk cells with cd56dim and cd56bright phenotype. Expression of cd56 on maturing myeloid cells or monocytes.
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